Inhibition by low concentrations of ouabain of prolactin-induced lactogenesis in rabbit mammary-gland explants
نویسندگان
چکیده
منابع مشابه
Properties of a prolactin receptor from the rabbit mammary gland.
Receptors for human, simian, ovine, bovine and murine prolactin, human growth hormone and human placental lactogen have been identified in plasma-membrane-containing subcellular particles isolated from rabbit mammary glands. The association and dissociation of (125)I-labelled prolactin are time- and temperature-dependent processes, both being maximal at 37 degrees C. (125)I-labelled prolactin p...
متن کاملTwo separate receptors for prolactin in the rabbit mammary gland.
Rabbit mammary gland PRL receptors in the microsome fraction were solubilized with the zwitterionic detergent Chaps, and were separated into two fractions (Fr. A and B) by ion-exchange chromatography. The number of receptors in Fr. B was about 2.2 times greater than in Fr. A. In sucrose gradient centrifugation analysis, PRL receptors in Fr. A and Fr. B sedimented at different positions. After b...
متن کاملDiet-induced obesity impairs mammary development and lactogenesis in murine mammary gland.
We have developed a mouse model of diet-induced obesity that shows numerous abnormalities relating to mammary gland function. Animals ate approximately 40% more calories when offered a high-fat diet and gained weight at three times the rate of controls. They exhibited reduced conception rates, increased peripartum pup mortality, and impaired lactogenesis. The impairment of lactogenesis involved...
متن کاملIdentification of prolactin-induced transcription factors in the mammary gland
a number of biological processes in vertebrates, including reproduction, growth and development, osmoregulation, metabolism and immunoregulation (Nicoll, 1974). The biological actions of prolactin have been most extensively characterized in the mammary gland, where it has an essential role both in the differentiation of the gland during pregnancy and in the regulation of milk protein gene expre...
متن کاملEffect of prolactin on phosphate transport and incorporation in mouse mammary gland explants.
Inorganic phosphate is present in milk at a concentration that is severalfold higher than in maternal plasma. In cultured mammary tissues from 12- to 14-day-pregnant mice, the intracellular concentration of (32)PO(4) was six times higher than in the culture medium after a 4-h treatment with (32)PO(4). Of the principal lactogenic hormones [insulin (I), cortisol (H), and prolactin (PRL)], only I ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1978
ISSN: 0264-6021
DOI: 10.1042/bj1720509